1Y44
Crystal structure of RNase Z
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9766, 0.9796, 0.9797 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.980, 188.330, 177.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.780 - 2.100 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.24800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.061 | 0.415 |
| Number of reflections | 85742 | |
| <I/σ(I)> | 8 | 0.49 |
| Completeness [%] | 79.3 | |
| Redundancy | 2.32 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG MME 2000, ammonium sulfate, sodium-MES, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 6 |
