1Y0V
Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-11 |
Detector | MARRESEARCH |
Wavelength(s) | 0.92 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 317.509, 183.348, 141.812 |
Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
Resolution | 29.870 - 3.600 |
R-factor | 0.286 |
Rwork | 0.286 |
R-free | 0.30700 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.730 |
High resolution limit [Å] | 3.100 | 3.600 |
Rmerge | 0.047 | 0.555 |
Number of reflections | 375293 | |
<I/σ(I)> | 20 | 2.1 |
Completeness [%] | 98.0 | 100 |
Redundancy | 3.8 | 3.02 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 277 | PEG400, MgCl2, Tris-HCl, pH 8.5, VAPOR DIFFUSION, temperature 277K |