1XZQ
Structure of the GTP-binding protein TrmE from Thermotoga maritima complexed with 5-formyl-THF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Wavelength(s) | 0.934 |
Spacegroup name | P 62 |
Unit cell lengths | 130.030, 130.030, 113.840 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.930 - 2.900 |
R-factor | 0.256 |
Rwork | 0.250 |
R-free | 0.30700 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.930 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.047 | 0.280 |
Number of reflections | 24138 | |
<I/σ(I)> | 25.08 | 7.3 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 6.3 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | ammonium sulphate, mes, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |