1XXH
ATPgS Bound E. Coli Clamp Loader Complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9641, 0.9797, 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.450, 106.460, 535.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 3.450 |
| Rwork | 0.315 |
| R-free | 0.35000 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.530 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Number of reflections | 73704 | |
| <I/σ(I)> | 15.1 | 2.1 |
| Completeness [%] | 97.8 | 86.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.95 | 293 | PEG 3350, Sodium Phosphate, Anapoe X-405, pH 7.95, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
