1XW9
Drosophila thioredoxin, oxidized, P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.741, 65.507, 62.075 |
Unit cell angles | 90.00, 113.09, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
Rwork | 0.219 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1auc |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 20371 | |
<I/σ(I)> | 21.4 | |
Completeness [%] | 98.4 | 95.6 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 298 | ammonium sulfate, PEG4000, pH 6.0, VAPOR DIFFUSION, temperature 298K |