1XW7
Diabetes-Associated Mutations in Human Insulin: Crystal Structure and Photo-Cross-Linking Studies of A-Chain Variant Insulin Wakayama
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-23 |
Detector | BRUKER |
Spacegroup name | P 21 3 |
Unit cell lengths | 70.852, 70.852, 70.852 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.100 - 2.300 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zeg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.100 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.065 | 0.066 |
Number of reflections | 5346 | |
<I/σ(I)> | 9.7 | 9.6 |
Completeness [%] | 97.7 | 95.2 |
Redundancy | 12.9 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.2 | 290 | Tris, sodium citrate, acetone, phenol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 6.20 |