1XU7
Crystal Structure of the Interface Open Conformation of Tetrameric 11b-HSD1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Detector technology | CCD |
| Collection date | 2003-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.3404, 1.3408, 1.3412, 1.2782 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.430, 159.625, 73.544 |
| Unit cell angles | 90.00, 93.07, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.19796 |
| Rwork | 0.197 |
| R-free | 0.21716 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.265 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 114558 | |
| <I/σ(I)> | 26.2 | 3 |
| Completeness [%] | 95.3 | 76.2 |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG , MES buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
