1XU7
Crystal Structure of the Interface Open Conformation of Tetrameric 11b-HSD1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Detector technology | CCD |
Collection date | 2003-06-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.3404, 1.3408, 1.3412, 1.2782 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.430, 159.625, 73.544 |
Unit cell angles | 90.00, 93.07, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.19796 |
Rwork | 0.197 |
R-free | 0.21716 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.265 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 114558 | |
<I/σ(I)> | 26.2 | 3 |
Completeness [%] | 95.3 | 76.2 |
Redundancy | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG , MES buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |