1XU2
The crystal structure of APRIL bound to BCMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-14 |
| Detector | SBC-3 |
| Wavelength(s) | 0.98040 |
| Spacegroup name | P 61 |
| Unit cell lengths | 114.294, 114.294, 91.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.18151 |
| Rwork | 0.178 |
| R-free | 0.21313 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APRIL alone (1U5Z) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 28292 | |
| <I/σ(I)> | 11.7 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 292 | well solution: 0.1M MES, 5% Peg 8000, 10% Peg 1000, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
