1XU1
The crystal structure of APRIL bound to TACI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-14 |
| Detector | SBC-3 |
| Wavelength(s) | 0.98040 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.339, 91.839, 102.268 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.17087 |
| Rwork | 0.167 |
| R-free | 0.20317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APRIL alone pdbcode 1U5Z |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.347 | |
| Number of reflections | 44579 | |
| <I/σ(I)> | 7.4 | 3.1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | well solution: 70% MPD, 0.1 M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
