1XTU
Sulfolobus solfataricus uracil phosphoribosyltransferase in complex with uridine 5'-monophosphate (UMP) and cytidine 5'-triphosphate (CTP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-19 |
Detector | MARRESEARCH |
Wavelength(s) | 1.098 |
Spacegroup name | P 1 |
Unit cell lengths | 71.670, 76.510, 91.450 |
Unit cell angles | 109.00, 90.76, 115.40 |
Refinement procedure
Resolution | 24.750 - 2.800 |
Rwork | 0.222 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Sulfolobus solfataricus UPRTase PDB ID 1XTT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.910 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 40931 | |
<I/σ(I)> | 6.6 | 1.9 |
Completeness [%] | 98.5 | 97.7 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 4000, magnesium chloride, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |