1XTT
Sulfolobus solfataricus uracil phosphoribosyltransferase in complex with uridine 5'-monophosphate (UMP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-03-22 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08535 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.840, 97.480, 73.440 |
Unit cell angles | 90.00, 93.73, 90.00 |
Refinement procedure
Resolution | 24.430 - 1.800 |
Rwork | 0.193 |
R-free | 0.22800 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 72112 | |
<I/σ(I)> | 12.7 | 3.3 |
Completeness [%] | 94.2 | 90.9 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 4000, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |