1XRH
Crystal Structure of Ureidoglycolate Dehydrogenase from Escherichia Coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-31 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 104.715, 129.916, 106.468 |
Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
Resolution | 19.860 - 2.250 |
R-factor | 0.1921 |
Rwork | 0.192 |
R-free | 0.25000 |
Structure solution method | SAD |
Starting model (for MR) | Density modified experimental electron density map based on Se sites. Se sites were obtained from poor phases from a weak MR solution using a model derived from pdb entry 1RFM. |
RMSD bond length | 0.018 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.860 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 213051 | |
<I/σ(I)> | 6.5 | |
Completeness [%] | 80.5 | 72 |
Redundancy | 1.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 15% PEG 4000, 0.1M Sodium Citrate pH 5.6, 0.06M Amonium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |