1XR2
Crystal Structure of oxidized T. maritima Cobalamin-Independent Methionine Synthase complexed with Methyltetrahydrofolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 164.058, 159.011, 64.543 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.350 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.800 * |
| Data scaling software | XDS |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 2.540 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 69289 | |
| <I/σ(I)> | 11.54 | 4.5 |
| Completeness [%] | 97.5 | 98.4 |
| Redundancy | 4.84 | 4.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 4.6 * | 295 | PEG 4000, SODIUM ACETATE, AMMONIUM SULFATE, pH 5.2, VAPOR BATCH, UNDER OIL, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | PEG4000 | 25 (%) | |
| 3 | 1 | 1 | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | 1 | sodium acetate | 0.1 (M) | pH4.6 |
| 5 | 1 | 2 | PEG4000 | 12 (%) | |
| 6 | 1 | 2 | ammonium sulfate | 0.2 (M) | |
| 7 | 1 | 2 | sodium acetate | 0.1 (M) | pH4.6 |
