1XR1
Crystal structure of hPim-1 kinase in complex with AMP-PNP at 2.1 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.680, 96.680, 80.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.430 - 2.100 |
| Rwork | 0.267 |
| R-free | 0.30100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.400 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.075 |
| Number of reflections | 40217 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 30% PEG1500, 0.1 M Hepes pH 7.5, 0.3 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 25K |
