1XQP
Crystal structure of 8-oxoguanosine complexed Pa-AGOG, 8-oxoguanine DNA glycosylase from Pyrobaculum aerophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-11-14 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 72.220, 98.110, 36.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.690 |
R-factor | 0.18467 |
Rwork | 0.183 |
R-free | 0.22479 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.016 |
RMSD bond angle | 1.609 |
Data scaling software | XDS |
Phasing software | PHASES |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.740 | 1.800 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.062 | |
Number of reflections | 28623 | |
<I/σ(I)> | 17 | 3.47 |
Completeness [%] | 96.7 | 90.5 |
Redundancy | 6.8 | 6.12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | PEG 4000, HEPES-NaOH, NaCl, Dithio threitol, Dimethyl sulfoxide, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |