1XPG
Crystal Structure of T. maritima Cobalamin-Independent Methionine Synthase complexed with Zn2+ and Methyltetrahydrofolate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2003-11-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 163.948, 159.303, 64.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.590 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.600 * |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.990 | 2.750 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 52850 | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 95.8 | 95.7 |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 4.6 * | 295 | PEG 4000, SODIUM ACETATE, AMMONIUM SULFATE, pH 5.2, vapor batch, under oil, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | PEG4000 | 25 (%) | |
| 3 | 1 | 1 | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | 1 | sodium acetate | 0.1 (M) | pH4.6 |
| 5 | 1 | 2 | PEG4000 | 12 (%) | |
| 6 | 1 | 2 | ammonium sulfate | 0.2 (M) | |
| 7 | 1 | 2 | sodium acetate | 0.1 (M) | pH4.6 |
