1XPG

Crystal Structure of T. maritima Cobalamin-Independent Methionine Synthase complexed with Zn2+ and Methyltetrahydrofolate

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Temperature [K]	295
Detector technology	CCD
Collection date	2003-11-16
Detector	MAR CCD 165 mm
Wavelength(s)	0.97920
Spacegroup name	P 21 21 2
Unit cell lengths	163.948, 159.303, 64.449
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.590
R-factor	0.196
Rwork	0.196
R-free	0.24800
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	22.600 *
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.990	2.750
High resolution limit [Å]	2.590	2.590
Number of reflections	52850
<I/σ(I)>	10.9
Completeness [%]	95.8	95.7
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	4.6 *	295	PEG 4000, SODIUM ACETATE, AMMONIUM SULFATE, pH 5.2, vapor batch, under oil, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	PEG4000	25 (%)
3	1	1	ammonium sulfate	0.2 (M)
4	1	1	sodium acetate	0.1 (M)	pH4.6
5	1	2	PEG4000	12 (%)
6	1	2	ammonium sulfate	0.2 (M)
7	1	2	sodium acetate	0.1 (M)	pH4.6