1XOZ
Catalytic Domain Of Human Phosphodiesterase 5A In Complex With Tadalafil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-09-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.102, 76.397, 80.699 |
| Unit cell angles | 90.00, 103.18, 90.00 |
Refinement procedure
| Resolution | 79.060 - 1.370 |
| R-factor | 0.1541 |
| Rwork | 0.152 |
| R-free | 0.19335 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.920 |
| Data scaling software | ELVES |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.25) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.060 | 1.406 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.077 | 0.894 |
| Number of reflections | 64263 | |
| <I/σ(I)> | 6.7 | 1.4 |
| Completeness [%] | 97.4 | 96.2 |
| Redundancy | 3.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | Jeffamine ED-2001, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
