1XMF
Structure of Mn(II)-Soaked Apo Methane Monooxygenase Hydroxylase Crystals from M. capsulatus (Bath)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-05-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.820, 171.679, 220.275 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.760 - 2.320 |
R-factor | 0.2191 |
Rwork | 0.219 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 1FZ1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.058 | 0.295 |
Number of reflections | 117119 | |
<I/σ(I)> | 11.3 | 2.8 |
Completeness [%] | 91.7 | 90.7 |
Redundancy | 2.3 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | MOPS, CaCl2, PEG8000, glycerol, azide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |