1XKY
Crystal Structure of Dihydrodipicolinate Synthase DapA-2 (BA3935) from Bacillus Anthracis at 1.94A Resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54189 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.203, 126.882, 173.319 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.940 |
| R-factor | 0.15555 |
| Rwork | 0.153 |
| R-free | 0.19719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dhp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.183 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.082 | 0.315 |
| Number of reflections | 87118 | |
| <I/σ(I)> | 17.2 | 3 |
| Completeness [%] | 95.6 | 84.7 |
| Redundancy | 5.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG 5000 MME, KSCN, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
