1XKL
Crystal Structure of Salicylic Acid-binding Protein 2 (SABP2) from Nicotiana tabacum, NESG Target AR2241
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97935 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 136.457, 167.773, 44.831 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.150 - 2.000 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.23400 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.200 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.075 | 0.290 |
Number of reflections | 131338 | |
<I/σ(I)> | 19.5 | 2.9 |
Completeness [%] | 96.6 | 82.2 |
Redundancy | 5.6 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM HEPES-NaOH, 20% PEG3350, 5 mM SA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277 K |