1XKG
Crystal structure of the major house dust mite allergen Der p 1 in its pro form at 1.61 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.659, 66.557, 73.388 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.610 |
| R-factor | 0.154 |
| Rwork | 0.153 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yal |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.486 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 ((MOLREP)) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.670 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Number of reflections | 41444 | |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 99.6 | 96.9 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | PEG-4000, ammonium sulfate, sodium acetate, glycerol, yttrium chloride, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
