1XK1
Crystal Structures of the G139A, G139A-NO and G143H Mutants of Human Heme Oxygenase-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.53 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.103, 75.212, 107.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.080 |
| R-factor | 0.246 |
| Rwork | 0.271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qq8 |
| RMSD bond length | 0.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 44222 | |
| Completeness [%] | 99.8 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 273 | ammonium sulphate, hepes, hexane diol , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
