1XJW
The Structure of E. coli Aspartate Transcarbamoylase Q137A Mutant in The R-State
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1997-12-30 |
Detector | UCSD MARK III |
Wavelength(s) | 1.5418 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 122.116, 122.116, 155.928 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 2.710 |
R-factor | 0.178 |
Rwork | 0.170 |
R-free | 0.22300 |
Structure solution method | Refinement from pre-existing model |
Starting model (for MR) | 1d09 |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 7.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.087 | 0.329 |
Number of reflections | 35809 | |
<I/σ(I)> | 10.5 | 2.59 |
Completeness [%] | 89.0 | 85 |
Redundancy | 2.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 5.9 | 295 | 12 mg/ml Buffer 50 mM Maleic Acid, 3 mM Sodium Azide 1 mM N-Phosphonacetyl-L-Aspartate, pH 5.9, MICRODIALYSIS, temperature 295K |