1XG5
Structure of human putative dehydrogenase MGC4172 in complex with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.710, 124.669, 75.130 |
| Unit cell angles | 90.00, 97.23, 90.00 |
Refinement procedure
| Resolution | 41.560 - 1.530 |
| R-factor | 0.16226 |
| Rwork | 0.162 |
| R-free | 0.20638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1edo |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.648 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.560 | 1.570 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.087 | 0.558 |
| Number of reflections | 145577 | |
| <I/σ(I)> | 13 | 1.6 |
| Completeness [%] | 97.0 | 81.2 |
| Redundancy | 5.3 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 100mM Bis-Tris pH 5.5, 200mM Ammonium Acetate, 2% Glycerol (total 7%), 18% PEG 3350, 0.5mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
