1XET
Crystal structure of stilbene synthase from Pinus sylvestris, complexed with methylmalonyl CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.465, 111.190, 132.015 |
| Unit cell angles | 90.00, 93.13, 90.00 |
Refinement procedure
| Resolution | 22.580 - 2.000 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.22300 |
| Structure solution method | isomorphous replacement |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.580 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 96601 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 292 | PEG 4000, citric acid, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
