1XEJ

THE CRYSTAL STRUCTURES OF LYSOZYME AT VERY LOW LEVELS OF HYDRATION

Experimental procedure

Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1992-10-04
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	26.345, 55.885, 31.550
Unit cell angles	90.00, 109.98, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.194 *
Rwork	0.194
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	THE 38% RELATIVE HUMIDITY FORM I STRUCTURE
RMSD bond length	0.012
RMSD bond angle	0.044
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.144
High resolution limit [Å]	2.100	2.061
Rmerge	0.063	0.307
Total number of observations	13358 *
Number of reflections	4847
<I/σ(I)>	14.08	2.55
Completeness [%]	90.5	48.5
Redundancy	2.76	1.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6		pH 4.6