1XDU
Crystal structure of Aclacinomycin-10-hydroxylase (RdmB) in complex with Sinefungin (SFG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0500 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.758, 86.813, 117.395 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.720 - 2.700 |
| R-factor | 0.23059 |
| Rwork | 0.227 |
| R-free | 0.29513 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RdmB+SAM complex |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.201 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.700 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 8940 | |
| <I/σ(I)> | 12.2 | 4.6 |
| Completeness [%] | 98.6 | 99.5 |
| Redundancy | 9.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | ammonium acetate, PEG 4000, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
