1XD3
Crystal structure of UCHL3-UbVME complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-06-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.110, 49.290, 67.620 |
| Unit cell angles | 86.12, 75.03, 76.78 |
Refinement procedure
| Resolution | 23.760 - 1.450 |
| R-factor | 0.177 |
| Rwork | 0.166 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uch PDB ENTRY 1UBQ |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.760 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 93859 | |
| <I/σ(I)> | 27.2 | 2.8 |
| Completeness [%] | 98.0 | 95.5 |
| Redundancy | 5.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | PEG 4000, magnesium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
