1XCJ
Guanidinoacetate methyltransferase containing S-adenosylhomocysteine and guanidinoacetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-01 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.188, 41.403, 42.320 |
| Unit cell angles | 104.25, 112.67, 104.45 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.225 |
| Rwork | 0.224 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.038 | |
| Number of reflections | 12785 | |
| Completeness [%] | 93.4 | 74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 8% PEG8000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
