1XBA
Crystal structure of apo syk tyrosine kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Detector technology | CCD |
| Collection date | 2002-11-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.785, 84.721, 90.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.830 - 2.000 |
| Rwork | 0.231 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.584 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.861 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.117 | 0.845 |
| Number of reflections | 21184 | |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 6.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 15% peg 3350, 100mM Tris HCl (pH 8.5) |
