1XB4
Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Wavelength(s) | .97914 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.360, 123.660, 140.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 3.100 |
R-factor | 0.273 |
Rwork | 0.327 |
R-free | 0.32700 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.400 |
High resolution limit [Å] | 3.050 |
Number of reflections | 18350 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG 8000, Magnesium acetate, glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG 8000, Magnesium acetate, glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |