1X6M
Crystal structure of the glutathione-dependent formaldehyde-activating enzyme (Gfa)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97980 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.360, 118.860, 95.990 |
Unit cell angles | 90.00, 97.48, 90.00 |
Refinement procedure
Resolution | 74.290 - 2.350 |
R-factor | 0.19348 |
Rwork | 0.189 |
R-free | 0.25022 |
Structure solution method | Se-SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.987 |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.330 | 2.450 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.077 | 0.383 |
Number of reflections | 49354 | |
<I/σ(I)> | 8.66 | 2.17 |
Completeness [%] | 99.8 | 100 |
Redundancy | 1.95 | 1.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | (NH4)2SO4, PEG 400, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |