1WZU
Crystal structure of quinolinate synthase (nadA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-23 |
Detector | ADSC |
Wavelength(s) | 1 |
Spacegroup name | P 63 |
Unit cell lengths | 94.610, 94.610, 62.570 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.500 - 2.000 |
R-factor | 0.222 |
Rwork | 0.217 |
R-free | 0.26800 |
Structure solution method | MIRAS |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 81.650 |
High resolution limit [Å] | 1.900 |
Number of reflections | 25173 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 18% PEG 600. 0.2M imidazole, 0.1M malate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |