1WYU
Crystal structure of glycine decarboxylase (P-protein) of the glycine cleavage system, in holo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2004-06-12 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 134.874, 166.673, 189.007 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.570 - 2.100 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.22900 |
Structure solution method | SIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.124 | 0.673 |
Number of reflections | 245553 | |
<I/σ(I)> | 15 | 2.6 |
Completeness [%] | 99.0 | 96.2 |
Redundancy | 6.9 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 3350, lithium sulfate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |