1WY9
Crystal structure of microglia-specific protein, Iba1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 44.056, 44.056, 99.144 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.610 - 2.100 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.25100 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.610 | 2.230 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 6506 | |
Completeness [%] | 93.7 | 75.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.4M sodium citrate in 100mM HEPES-Na (pH 7.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.4M sodium citrate in 100mM HEPES-Na (pH 7.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.4M sodium citrate in 100mM HEPES-Na (pH 7.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K |