1WY7
crystal structure of a putative RNA methyltransferase PH1948 from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.975, 43.141, 118.240 |
| Unit cell angles | 90.00, 92.08, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| Rwork | 0.242 |
| R-free | 0.26800 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.390 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.860 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.046 | 0.218 |
| Number of reflections | 53514 | |
| <I/σ(I)> | 20 | 5.6 |
| Completeness [%] | 99.3 | 94.9 |
| Redundancy | 5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | PEG400, HEPES-Na, CaCl2, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
