1WVF
p-Cresol Methylhydroxylase: Alteration of the Structure of the Flavoprotein Subunit upon its Binding to the Cytochrome Subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-27 |
| Detector | SBC |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.640, 116.910, 50.220 |
| Unit cell angles | 90.00, 113.18, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.300 |
| Rwork | 0.146 |
| R-free | 0.16700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1DII |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.630 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.089 | 0.155 |
| Number of reflections | 100440 | |
| <I/σ(I)> | 19.6 | 4.1 |
| Completeness [%] | 87.3 | 46.1 |
| Redundancy | 4.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 296 | PEG 1500, acetate, magnesium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
