1WUR

Structure of GTP cyclohydrolase I Complexed with 8-oxo-dGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-28
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	160.570, 110.560, 70.530
Unit cell angles	90.00, 105.78, 90.00

Refinement procedure

Resolution	46.690 - 1.820
Rwork	0.206
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.890
High resolution limit [Å]	1.820	1.820
Rmerge	0.050
Number of reflections	103478
<I/σ(I)>	25
Completeness [%]	98.5	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	3.4% PEG400, 0.1M HEPES, 2.0M Ammonium sulfate, 15% glycerol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K