1WSV
Crystal Structure of Human T-protein of Glycine Cleavage System
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-30 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.670, 129.060, 135.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.530 - 2.600 |
R-factor | 0.171 |
Rwork | 0.171 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wsr |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.113 | 0.421 |
Number of reflections | 28804 | |
<I/σ(I)> | 14.5 | 3.7 |
Completeness [%] | 95.3 | 90.3 |
Redundancy | 5.2 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | ammonium sulfate, potassium sodium tartrate, citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |