1WQ6
The tetramer structure of the nervy homolgy two (NHR2) domain of AML1-ETO is critical for AML1-ETO'S activity
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97946, 0.97915, 0.96422 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.720, 75.442, 30.781 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.21575 |
Rwork | 0.213 |
R-free | 0.27012 |
Structure solution method | MAD |
RMSD bond length | 0.021 |
RMSD bond angle | 2.794 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.044 | 0.196 |
Number of reflections | 10096 | |
<I/σ(I)> | 24.9 | 5.5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 6.7 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 100mM Na citrate (pH 5.6), 100mM MgCl2, 30% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K |