1WPO
HYDROLYTIC ENZYME HUMAN CYTOMEGALOVIRUS PROTEASE
Experimental procedure
Spacegroup name | P 41 21 2 |
Unit cell lengths | 71.000, 71.000, 209.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.28800 |
RMSD bond length | 0.010 |
RMSD bond angle | 24.200 * |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 25 (mg/ml) | |
2 | 1 | 1 | sodium acetate | 20 (mM) | |
3 | 1 | 1 | 80 (mM) | ||
4 | 1 | 1 | dithiothreitol | 1 (mM) | |
5 | 1 | 2 | PEG4000 | 16 (%) | |
6 | 1 | 2 | MES | 0.1 (M) | |
7 | 1 | 2 | 0.4 (M) | ||
8 | 1 | 2 | glycerol | 10 (%) | |
9 | 1 | 2 | t-butanol | 5 (%) |