1WNT
Structure of the tetrameric form of Human L-Xylulose Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.941, 59.960, 82.805 |
| Unit cell angles | 90.00, 91.68, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.300 |
| R-factor | 0.2198 |
| Rwork | 0.202 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | XR dimer (1PR9) |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.286 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 32262 | |
| <I/σ(I)> | 1.98 | |
| Completeness [%] | 92.0 | 90.9 |
| Redundancy | 1.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG 4000, Sodium Citrate, Isopropanol, Urea, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
