1WN5
Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Cacodylic Acid
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.0200, 1.2818, 1.2822 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.672, 69.293, 144.714 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.610 - 1.800 |
| R-factor | 0.197 |
| Rwork | 0.189 |
| R-free | 0.22000 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.590 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.057 |
| Number of reflections | 51195 |
| Completeness [%] | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
