1WKX
Crystal Structure of a Hev b 6.02 Isoallergen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-23 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 37.200, 37.200, 48.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.265 - 1.700 |
| R-factor | 0.156 |
| Rwork | 0.152 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q9b |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.396 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.270 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.037 | 0.209 |
| Number of reflections | 4440 | |
| <I/σ(I)> | 25.2 | 4.5 |
| Completeness [%] | 97.1 | 90.5 |
| Redundancy | 3.9 | 3.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 291 | MPD, Tris, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
