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1Q9B

CRYSTAL STRUCTURE ANALYSIS OF Hev b 6.02 (HEVEIN) AT 1.5 ANGSTROMS RESOLUTION

Summary for 1Q9B
Entry DOI10.2210/pdb1q9b/pdb
NMR InformationBMRB: 6123
DescriptorHevein, (4S)-2-METHYL-2,4-PENTANEDIOL (3 entities in total)
Functional Keywordsallergen, lectin, agglutinin-toxin motif
Biological sourceHevea brasiliensis
Total number of polymer chains1
Total formula weight4967.51
Authors
Rodriguez-Romero, A.,Hernandez-Santoyo, A. (deposition date: 2003-08-22, release date: 2004-01-13, Last modification date: 2023-08-16)
Primary citationReyes-Lopez, C.A.,Hernandez-Santoyo, A.,Pedraza-Escalona, M.,Mendoza, G.,Hernandez-Arana, A.,Rodriguez-Romero, A.
Insights into a conformational epitope of Hev b 6.02 (hevein).
Biochem.Biophys.Res.Commun., 314:123-130, 2004
Cited by
PubMed Abstract: Hevein (Hev b 6.02) is a major IgE-binding allergen in natural rubber latex and manufactured products. Both tryptophans (Trp(21) and Trp(23)) of the hevein molecule were chemically modified with BNPS-skatole (2-nitrophenylsulfenyl-3-methyl-3(')-bromoindolenine); derivatized allergen failed to significantly inhibit binding of serum IgE in ELISA assays. Similarly, skin prick tests showed that hevein-positive patients gave no response with the modified allergen. Dot blot experiments carried out with anti-hevein mono- and polyclonal antibodies confirmed the importance of Trp(21) and Trp(23) for antibody-recognition, and demonstrated the specific cross-reactivity of other molecules containing hevein-like domains. We also report the structure of Hev b 6.02 at an extended resolution (1.5A) and compare its surface properties around Trp residues with those of similar regions in other allergens. Overall our results indicate that the central part of the protein, which comprises three aromatic and other acidic and polar residues, constitutes a conformational epitope.
PubMed: 14715255
DOI: 10.1016/j.bbrc.2003.12.068
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.5 Å)
Structure validation

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