1Q9B
CRYSTAL STRUCTURE ANALYSIS OF Hev b 6.02 (HEVEIN) AT 1.5 ANGSTROMS RESOLUTION
Summary for 1Q9B
Entry DOI | 10.2210/pdb1q9b/pdb |
NMR Information | BMRB: 6123 |
Descriptor | Hevein, (4S)-2-METHYL-2,4-PENTANEDIOL (3 entities in total) |
Functional Keywords | allergen, lectin, agglutinin-toxin motif |
Biological source | Hevea brasiliensis |
Total number of polymer chains | 1 |
Total formula weight | 4967.51 |
Authors | Rodriguez-Romero, A.,Hernandez-Santoyo, A. (deposition date: 2003-08-22, release date: 2004-01-13, Last modification date: 2023-08-16) |
Primary citation | Reyes-Lopez, C.A.,Hernandez-Santoyo, A.,Pedraza-Escalona, M.,Mendoza, G.,Hernandez-Arana, A.,Rodriguez-Romero, A. Insights into a conformational epitope of Hev b 6.02 (hevein). Biochem.Biophys.Res.Commun., 314:123-130, 2004 Cited by PubMed Abstract: Hevein (Hev b 6.02) is a major IgE-binding allergen in natural rubber latex and manufactured products. Both tryptophans (Trp(21) and Trp(23)) of the hevein molecule were chemically modified with BNPS-skatole (2-nitrophenylsulfenyl-3-methyl-3(')-bromoindolenine); derivatized allergen failed to significantly inhibit binding of serum IgE in ELISA assays. Similarly, skin prick tests showed that hevein-positive patients gave no response with the modified allergen. Dot blot experiments carried out with anti-hevein mono- and polyclonal antibodies confirmed the importance of Trp(21) and Trp(23) for antibody-recognition, and demonstrated the specific cross-reactivity of other molecules containing hevein-like domains. We also report the structure of Hev b 6.02 at an extended resolution (1.5A) and compare its surface properties around Trp residues with those of similar regions in other allergens. Overall our results indicate that the central part of the protein, which comprises three aromatic and other acidic and polar residues, constitutes a conformational epitope. PubMed: 14715255DOI: 10.1016/j.bbrc.2003.12.068 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.5 Å) |
Structure validation
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