1WG3
Structural analysis of yeast nucleosome-assembly factor CIA1p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2001-10-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.629, 47.048, 40.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.420 - 3.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1roc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DPS |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.130 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.085 | 0.287 |
| Number of reflections | 4606 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 99.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG8K, AS, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
