1WE5

Crystal Structure of Alpha-Xylosidase from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-12-08
Detector	MACSCIENCE
Wavelength(s)	0.90000
Spacegroup name	P 21 21 21
Unit cell lengths	161.337, 174.791, 209.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
Rwork	0.205
R-free	0.24730
Structure solution method	Crystal 1 SINGLE WAVELENGTH PROTOCOL, Crysatl 2 MAD PROTOCOL
RMSD bond length	0.007
RMSD bond angle	1.401
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	228852
<I/σ(I)>	10
Completeness [%]	99.2	95.6
Redundancy	5.2	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	PEG 20000, MES, glycerol, isopropanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	PEG 20000, MES, glycerol, isopropanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K