1WDC
SCALLOP MYOSIN REGULATORY DOMAIN
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-10 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.650, 87.100, 55.500 |
| Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.28100 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 24.135 * |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.020 |
| Rmerge | 0.061 |
| Total number of observations | 104023 * |
| Number of reflections | 26725 * |
| Completeness [%] | 89.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 4 * | Xie, X., (1994) Nature, 368, 306. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | drop | 0.1 (mM) | ||
| 4 | 1 | drop | HEPES | 75 (mM) | |
| 5 | 1 | drop | PEG4000 | 7-8 (%) | |
| 6 | 1 | reservoir | PEG4000 | 14-16 (%(w/v)) |
