1WCX
Crystal Structure of Mutant Uroporphyrinogen III Synthase from an Extremely Thermophilic Bacterium Thermus thermophilus HB8 (L75M/I193M/L248M, SeMet derivative, Form-1 crystal)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.548, 66.105, 55.857 |
| Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
| Resolution | 42.660 - 2.000 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.22500 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
