1WCS
A mutant of Trypanosoma rangeli sialidase displaying trans-sialidase activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-12-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 94.691, 94.691, 156.335 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 158.110 - 2.800 |
| R-factor | 0.248 |
| Rwork | 0.244 |
| R-free | 0.33600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n1s |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.429 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.510 |
| Number of reflections | 17626 | |
| Completeness [%] | 98.4 | 98.2 |
| Redundancy | 6.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 16% PEG-8000, 50MM SODIUM CACODYLATE, 100 MM AMMONIUM SULFATE, PH 6.5 |
